| Primary Identifier | MGI:6690849 | Allele Type | Targeted |
| Attribute String | Null/knockout, Reporter | Gene | Tmc8 |
| Transmission | Germline | Strain of Origin | 129S6/SvEv |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A 1.6-kb fragment containing exons 6-9 was replaced with a nuclear localization signal and LacZ reporter gene, the Engrailed-2 intron and splice acceptor site (En-2/SA), and an internal ribosome entry site followed by a loxP-flanked phosphoglycerine kinase (PGK)-neomycin resistance gene. The loxP-flanked PGK-neomycin resistance gene was removed via Cre-mediated recombination in the germline. Western blot analysis using a monoclonal anti-TMC8 antibody confirmed the complete absence of protein expression in cell lysates from homozygous mutant thymocytes. |
