| First Author | Villalon E | Year | 2014 |
| Journal | Methods Mol Biol | Volume | 1092 |
| Pages | 81-94 | PubMed ID | 24318815 |
| Mgi Jnum | J:210188 | Mgi Id | MGI:5569693 |
| Doi | 10.1007/978-1-60327-292-6_6 | Citation | Villalon E, et al. (2014) Real-time PCR quantification of gene expression in embryonic mouse tissue. Methods Mol Biol 1092:81-94 |
| abstractText | The Gbx family of transcription factors consists of two closely related proteins GBX1 and GBX2. A defining feature of the GBX family is a highly conserved 60 amino acid DNA-binding domain, which differs by just two amino acids. Gbx1 and Gbx2 are co-expressed in several areas of the developing central nervous system including the forebrain, anterior hindbrain, and spinal cord, suggesting the potential for genetic redundancy. However, there is a spatiotemporal difference in expression of Gbx1 and Gbx2 in the forebrain and spinal cord. Gbx2 has been shown to play a critical role in positioning the midbrain/hindbrain boundary and developing anterior hindbrain, whereas gene-targeting experiments in mice have revealed an essential function for Gbx1 in the spinal cord for normal locomotion. To determine if Gbx2 could potentially compensate for a loss of Gbx1 in the developing spinal cord, we performed real-time PCR to examine levels of Gbx2 expression in Gbx1(-/-) spinal cord at embryonic day (E) 13.5, a developmental stage when Gbx2 is rapidly downregulated. We demonstrate that Gbx2 expression is elevated in the spinal cord of Gbx1(-/-) embryos. |
