| Primary Identifier | MGI:3044616 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Tcf7l1 |
| Transmission | Germline | Strain of Origin | 129/Sv |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A loxP flanked 64 bp segment of exon 2 and a neo cassette were inserted via homologous recombination then removed via cre mediated recombination prior to generation of chimeric mice. The resulting allele lacked exon 2 and contained a frameshift and early termination codon. Immunoblot analysis confirmed the absence of protein expression in homozygous embryos |
