| First Author | Salaheldeen E | Year | 2012 |
| Journal | Mol Reprod Dev | Volume | 79 |
| Issue | 12 | Pages | 843-52 |
| PubMed ID | 23070997 | Mgi Jnum | J:190991 |
| Mgi Id | MGI:5451130 | Doi | 10.1002/mrd.22123 |
| Citation | Salaheldeen E, et al. (2012) Molecular cloning and localization of a CEACAM2 isoform, CEACAM2-L, expressed in spermatids in mouse testis. Mol Reprod Dev 79(12):843-52 |
| abstractText | Carcinoembryonic antigen (CEA) family, a subgroup of the immunoglobulin (Ig) superfamily, is divided into two sub-families: the CEA-related cell adhesion molecules (CEACAM) and the pregnancy-specific glycoproteins. The isoform CEACAM2 is expressed in mouse testis; in this study, we identified a novel isoform of Ceacam2, Ceacam2-Long (Ceacam2-L). CEACAM2-L is different from CEACAM2 in that it has much longer cytoplasmic tail region. Ceacam2-L starts to appear faintly in mouse testis after 3 weeks of postnatal development, and its expression level increased after 5 weeks. Immunoblot analysis confirmed the expression of CEACAM2-L in the seminiferous epithelium of mouse testis. Immunohistochemical data showed that CEACAM2-L was not observed on spermatogonia, spermatocytes, round spermatids, or Sertoli cells, but was seen at the plasma membrane of elongating spermatids in contact with extended cytoplasmic processes of Sertoli cells. CEACAM2-L was not detected at the head region of elongating spermatids, where the apical ectoplasmic specialization is constructed. These data suggest that CEACAM2-L might be a novel adhesion molecule contributing to cell-to-cell adhesion between elongating spermatids and Sertoli cells within the seminiferous epithelium. Mol. Reprod. Dev. 79: 843-852, 2012. (c) 2012 Wiley Periodicals, Inc. |
