Primary Identifier | MGI:6160939 | Allele Type | Transgenic |
Attribute String | Inducible, Recombinase, Reporter | Gene | Tg(hs660-cre/ERT2,-GFP)847Jlr |
Strain of Origin | FVB/NJ | Induced With | tamoxifen |
Is Recombinase | true | Is Wild Type | false |
molecularNote | This transgene contains the human enhancer element hs660 -- which is flanked on human Chr 15 by the SMAD family member 6 (SMAD6) and SMAD family member 3 (SMAD3) loci -- followed by a mouse minimal Hsp68 promoter, the cre/ERT2 fusion gene, an internal ribosomal entry site (IRES), and a green fluorescent protein reporter gene. The fusion gene encodes Cre recombinase joined to a modified ligand-binding domain of the human estrogen receptor that preferentially binds tamoxifen or 4-hydroxytamoxifen, but not endogenous 17beta-estradiol; upon binding to tamoxifen, the cre/ERT2 protein is translocated to the nucleus. |