| First Author | Laskowski AI | Year | 2013 |
| Journal | Biochem Biophys Res Commun | Volume | 435 |
| Issue | 4 | Pages | 551-6 |
| PubMed ID | 23665319 | Mgi Jnum | J:202640 |
| Mgi Id | MGI:5520144 | Doi | 10.1016/j.bbrc.2013.04.100 |
| Citation | Laskowski AI, et al. (2013) Myc binds the pluripotency factor Utf1 through the basic-helix-loop-helix leucine zipper domain. Biochem Biophys Res Commun 435(4):551-6 |
| abstractText | In order to elucidate the function of Myc in the maintenance of pluripotency and self-renewal in mouse embryonic stem cells (mESCs), we screened for novel ESC-specific interactors of Myc by mass spectrometry. Undifferentiated embryonic cell transcription factor 1 (Utf1) was identified in the screen as a putative Myc binding protein in mESCs. We found that Myc and Utf1 directly interact. Utf1 is a chromatin-associated factor required for maintaining pluripotency and self-renewal in mESCs. It can also replace c-myc during induced pluripotent stem cell (iPSC) generation with relatively high efficiency, and shares target genes with Myc in mESCs highlighting a potentially redundant functional role between Myc and Utf1. A large region of Utf1 was found to be necessary for direct interaction with N-Myc, while the basic helix-loop-helix leucine zipper domain of N-Myc is necessary for direct interaction with Utf1. |
