Primary Identifier | MGI:7484513 | Allele Type | Targeted |
Attribute String | Conditional ready, No functional change, Reporter | Gene | Htr1a |
Transmission | Germline | Strain of Origin | 129S6/SvEvTac |
Is Recombinase | false | Is Wild Type | false |
molecularNote | The single exon and the 3â untranslated region containing the polyadenylation signal were flanked by loxP sites. A modified yellow fluorescent protein, YPet, cDNA was inserted downstream of the 3â loxP site such that when the floxed region is removed, YPet is expressed under the endogenous Htr1a promoter. An inserted FRT-flanked PGK-EM7-Neomycin minigene was removed via flp-mediated recombination. |