| First Author | Hwang TW | Year | 2020 |
| Journal | J Nutr Biochem | Volume | 82 |
| Pages | 108394 | PubMed ID | 32454411 |
| Mgi Jnum | J:298208 | Mgi Id | MGI:6477441 |
| Doi | 10.1016/j.jnutbio.2020.108394 | Citation | Hwang TW, et al. (2020) Fat-1 expression enhance hippocampal memory in scopolamine-induced amnesia. J Nutr Biochem 82:108394 |
| abstractText | Omega-3 polyunsaturated fatty acids (PUFA) are critical for optimal brain health and are involved in psychiatric and neurological ailments. Here, we report the effects of higher endogenous omega-3 PUFA on memory impairment in the hippocampus by studying mice with transgenic expression of the fat-1 gene that converts omega-6 to omega-3 PUFA. We performed Y-maze and passive avoidance tests to evaluate the memory function of fat-1 mice treated with scopolamine. Fat-1 mice showed induced alternation in the Y-maze test and increased latency in the passive avoidance test. The effects of scopolamine on hippocampal neurogenesis were confirmed by increases in the number of Ki-67- and DCX-positive cells in the fat-1 mice. Western blotting revealed increased brain-derived neurotrophic factor (BDNF) and phosphorylated cAMP response element-binding protein levels, and lower scopolamine-induced apoptosis based on the cleaved-caspase 3 protein level in fat-1 mice. These findings suggest that higher endogenous omega-3 PUFA prevented granular cell loss, increased BDNF signaling, and decreased apoptosis signaling in scopolamine-treated fat-1 mice. These processes may underlie granular cell survival and suggest potential therapeutic targets for memory impairment. |
