| First Author | Grisart B | Year | 2002 |
| Journal | Genome Res | Volume | 12 |
| Issue | 2 | Pages | 222-31 |
| PubMed ID | 11827942 | Mgi Jnum | J:74690 |
| Mgi Id | MGI:2158974 | Doi | 10.1101/gr.224202 |
| Citation | Grisart B, et al. (2002) Positional Candidate Cloning of a QTL in Dairy Cattle: Identification of a Missense Mutation in the Bovine DGAT1 Gene with Major Effect on Milk Yield and Composition. Genome Res 12(2):222-31 |
| abstractText | We recently mapped a quantitative trait locus (QTL) with a major effect on milk composition- particularly fat content-to the centromeric end of bovine chromosome 14. We subsequently exploited linkage disequilibrium to refine the map position of this QTL to a 3-cM chromosome interval bounded by microsatellite markers BULGE13 and BULGE09. We herein report the positional candidate cloning of this QTL, involving (1) the construction of a BAC contig spanning the corresponding marker interval, (2) the demonstration that a very strong candidate gene, acylCoA:diacylglycerol acyltransferase (DGAT1), maps to that contig, and (3) the identification of a nonconservative K232A substitution in the DGAT1 gene with a major effect on milk fat content and other milk characteristics. [The sequence data described in this paper have been submitted to the GenBank data library under accession number AY065621.] |
