| First Author | Gabig TG | Year | 1994 |
| Journal | J Biol Chem | Volume | 269 |
| Issue | 47 | Pages | 29515-9 |
| PubMed ID | 7961935 | Mgi Jnum | J:40256 |
| Mgi Id | MGI:87601 | Doi | 10.1016/s0021-9258(18)43910-5 |
| Citation | Gabig TG, et al. (1994) Requiem: a novel zinc finger gene essential for apoptosis in myeloid cells. J Biol Chem 269(47):29515-9 |
| abstractText | To identify genes mediating programmed cell death triggered by interleukin 3 (IL-3)-deprivation of myeloid cells, the IL-3-dependent murine myeloid cell line FDCP-1 was used to screen a mammalian cell expression library for cDNAs that would promote survival following withdrawal of IL-3. A unique 892-base pair cDNA was cloned that prevented the programmed cell death response following IL-3 deprivation by causing antisense suppression of an endogenous 2.4-kilobase (kb) mRNA. A 2.3-kb cDNA containing the identical 892-base pair over-lapping sequence was cloned that encoded a deduced 371-amino acid protein containing a single Kruppel-type zinc finger and a cluster of 4 cysteine/histidine-rich repeats resembling atypical zinc fingers. The 2.4-kb mRNA was found to be ubiquitously expressed in murine tissues and its abundance in FDCP-1 cells was not altered in response to IL-3 deprivation. Since expression of this 2.4-kb mRNA was a prerequisite for the apoptosis response following IL-3 deprivation, the gene encoding it was named requiem. Requiem is likely to encode a transcription factor required for the apoptosis response following survival factor withdrawal from myeloid cells. |
