Primary Identifier | MGI:5827553 | Allele Type | Endonuclease-mediated |
Attribute String | Null/knockout | Gene | Sting1 |
Strain of Origin | C57BL/6 | Is Recombinase | false |
Is Wild Type | false |
molecularNote | Exon 3 was targeted with the CRISPR/Cas9 system. The sgRNA sequence was 5'-CACCTAGCCTCGCACGAActt-3' (recognition sequence in upper case) and the experiment resulted in a 5 bp deletion (CTCGC) in the middle of the recognition sequence. The resulting frameshift introduces a premature stop codon soon after, knocking the gene out. Western blots confirmed the absence of peptides translated from this allele in homozygous mice. |