| First Author | Taranum S | Year | 2012 |
| Journal | Int J Cell Biol | Volume | 2012 |
| Pages | 736524 | PubMed ID | 22518138 |
| Mgi Jnum | J:187540 | Mgi Id | MGI:5437402 |
| Doi | 10.1155/2012/736524 | Citation | Taranum S, et al. (2012) Cytoskeletal interactions at the nuclear envelope mediated by nesprins. Int J Cell Biol 2012:736524 |
| abstractText | Nesprin-1 is a giant tail-anchored nuclear envelope protein composed of an N-terminal F-actin binding domain, a long linker region formed by multiple spectrin repeats and a C-terminal transmembrane domain. Based on this structure, it connects the nucleus to the actin cytoskeleton. Earlier reports had shown that Nesprin-1 binds to nuclear envelope proteins emerin and lamin through C-terminal spectrin repeats. These repeats can also self-associate. We focus on the N-terminal Nesprin-1 sequences and show that they interact with Nesprin-3, a further member of the Nesprin family, which connects the nucleus to the intermediate filament network. We show that upon ectopic expression of Nesprin-3 in COS7 cells, which are nearly devoid of Nesprin-3 in vitro, vimentin filaments are recruited to the nucleus and provide evidence for an F-actin interaction of Nesprin-3 in vitro. We propose that Nesprins through interactions amongst themselves and amongst the various Nesprins form a network around the nucleus and connect the nucleus to several cytoskeletal networks of the cell. |
