| Primary Identifier | MGI:6442576 | Allele Type | Targeted |
| Attribute String | Inserted expressed sequence, Lineage barcode, Reporter | Gene | Col1a1 |
| Transmission | Germline | Strain of Origin | (C57BL/6 x 129S4/SvJae)F1 |
| Is Recombinase | false | Is Wild Type | false |
| description | KH2 embryonic stem cells carrying the Gt(ROSA)26Sortm1(rtTA*M2)Jae allele, were previously re-targeted to insert an FRT-flanked PGK-Neo cassette and a promoterless hygromycin resistance cassette in the 3' UTR of the Col1a1 locus. |
| molecularNote | The targeting construct is designed as a multiplex containing 10 single guide RNAs (sgRNA) driven by separate U6 promoters inserted into the 3' UTR of an EGFP reporter driven by constitutive CAG promoter. A "barcode" 276 bp array containing target sites matching each of the expressed sgRNAs is inserted upstream of the EGFP polyadenylation site. |
