| Primary Identifier | MGI:6256960 | Allele Type | Endonuclease-mediated |
| Attribute String | Null/knockout | Gene | Del(3Bglap2-Bglap)1Vari |
| Strain of Origin | (C57BL/6 x C3H)F2 | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | CRISPR/Cas9 technology is used to delete exon 2 of Bglap through exon 4 of Bglap2 (6.8 kb). This deletion leaves the first 4 amino acids of exon 2 of Bglap intact followed by a 5bp insertion and a portion of exon 4 of Bglap2. The mutation is predicted to create a reading frame shift after the 25th amino acid and a termination codon 17 residues further downstream. Mature protein is undetectable in homozygous animals. |
