| First Author | Wick MJ | Year | 1995 |
| Journal | Gene | Volume | 152 |
| Issue | 2 | Pages | 227-32 |
| PubMed ID | 7835705 | Mgi Jnum | J:22659 |
| Mgi Id | MGI:70517 | Doi | 10.1016/0378-1119(94)00676-j |
| Citation | Wick MJ, et al. (1995) Isolation of a cDNA encoding a novel zinc-finger protein from neuroblastoma x glioma NG108-15 cells. Gene 152(2):227-32 |
| abstractText | A subtraction cDNA library was constructed from control hybrid NG108-15 (mouse neuroblastoma x rat glioma) cells and NG108-15 cells which had been treated for 48 h with the delta-opioid agonist D-Ala2-D-Leu5 enkephalin (DADLE) to down-regulate the delta-opioid receptor on these cells. Among the clones isolated from this library was NGD16-4, a 2768-bp clone encoding a putative 64-kDa protein containing 14 tandemly repeated zinc fingers (Zf) with high homology to the Kruppel family of Zf proteins. NGD16-4 also contains a region homologous to the A element of the Kruppel Associated Box (KRAB) domain, a domain recently linked to transcriptional repression. Southern and Northern analyses indicate that NGD16-4 is derived from the mouse genome. Northern analysis also demonstrates that expression of NGD16-4 mRNA is much higher in several mouse neuroblastoma cell lines than in mouse brain or other tissues. Although the function of NGD16-4 is unclear, the expression pattern of NGD16-4 indicates a possible association with the processes of differentiation or transformation in the mouse. |
