| First Author | Ussar S | Year | 2006 |
| Journal | Exp Cell Res | Volume | 312 |
| Issue | 16 | Pages | 3142-51 |
| PubMed ID | 16876785 | Mgi Jnum | J:114381 |
| Mgi Id | MGI:3688938 | Doi | 10.1016/j.yexcr.2006.06.030 |
| Citation | Ussar S, et al. (2006) The Kindlins: subcellular localization and expression during murine development. Exp Cell Res 312(16):3142-51 |
| abstractText | The three Kindlins are a novel family of focal adhesion proteins. The Kindlin-1 (URP1) gene is mutated in Kindler syndrome, the first skin blistering disease affecting actin attachment in basal keratinocytes. Kindlin-2 (Mig-2), the best studied member of this family, binds ILK and Migfilin, which links Kindlin-2 to the actin cytoskeleton. Kindlin-3 is expressed in hematopoietic cells. Here we describe the genomic organization, gene expression and subcellular localization of murine Kindlins-1 to -3. In situ hybridizations showed that Kindlin-1 is preferentially expressed in epithelia, and Kindlin-2 in striated and smooth muscle cells. Kindlins-1 and -2 are both expressed in the epidermis. While both localize to integrin-mediated adhesion sites in cultured keratinocytes Kindlin-2, but not Kindlin-1, colocalizes with E-cadherin to cell-cell contacts in differentiated keratinocytes. Using a Kindlin-3-specific antiserum and an EGFP-tagged Kindlin-3 construct, we could show that Kindlin-3 is present in the F-actin surrounding ring structure of podosomes, which are specialized adhesion structures of hematopoietic cells. |
