| First Author | Nudel U | Year | 1980 |
| Journal | Nucleic Acids Res | Volume | 8 |
| Issue | 10 | Pages | 2133-46 |
| PubMed ID | 7433088 | Mgi Jnum | J:6412 |
| Mgi Id | MGI:54888 | Doi | 10.1093/nar/8.10.2133 |
| Citation | Nudel U, et al. (1980) Identification of recombinant phages containing sequences from different rat myosin heavy chain genes. Nucleic Acids Res 8(10):2133-46 |
| abstractText | The construction and identification of a recombinant plasmid containing a cDNA insert which hybridizes specifically to myosin heavy chain mRNA is described. The plasmid was used as a probe to screen a rat genomic library for recombinant phages containing myosin heavy chain sequences. Six clones with approximately 15 k bp inserts each were isolated. Digestion with several restriction enzymes and hybridization of the fractionated DNA with the plasmid probe showed that the clones contained 3 different DNA inserts. Electron microscopy of a heteroduplex made by hybridization of DNA from two clones confirmed that the inserts originated in different genes. Hybridization of size-fractionated ECOR1 digested rat spleen DNA with the cloned probe suggested the existence of at least 5 myosin heavy chain genes. |
