| Primary Identifier | MGI:3577050 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Sema5a |
| Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A targeting vector was designed to replace exons 4 and 5 with a PGK-neo cassette. The null allele was achieved by inserting a stop codon after amino acid 121, thereby rendering a truncated protein unlikely to be biological active. Alternatively, aberrant splicing between exons 3 and 6 would result in the generation of a stop codon after amino acid 141. Western blot analysis of membrane fractions demonstrated a complete lack of protein in mutants. |
