| First Author | Guo J | Year | 2002 |
| Journal | Nat Immunol | Volume | 3 |
| Issue | 5 | Pages | 469-76 |
| PubMed ID | 11967541 | Mgi Jnum | J:76237 |
| Mgi Id | MGI:2178895 | Doi | 10.1038/ni791 |
| Citation | Guo J, et al. (2002) Regulation of the TCRalpha repertoire by the survival window of CD4(+)CD8(+) thymocytes. Nat Immunol 3(5):469-76 |
| abstractText | T cell receptor (TCR) alpha alleles undergo primary and secondary rearrangement in double-positive (DP) thymocytes. By analyzing TCRalpha rearrangement in orphan nuclear receptor RORgamma-deficient mice, in which the DP lifespan is shorter, and in Bcl-x(L)-transgenic mice, in which the DP lifespan is extended, we show that the progression of secondary V(alpha) to J(alpha) rearrangements is controlled by DP thymocyte survival. In addition, because Bcl-x(L) induces a bias towards 3' J(alpha) usage in peripheral T cells, we conclude that the programmed cell death of DP thymocytes is not simply a consequence of failed positive selection. Rather, it limits the progression of rearrangement along the J(alpha) locus and the opportunities for positive selection, thereby regulating the TCRalpha repertoire. |
