| First Author | Yang Q | Year | 1997 |
| Journal | Mol Cell Biol | Volume | 17 |
| Issue | 9 | Pages | 5236-43 |
| PubMed ID | 9271401 | Mgi Jnum | J:42857 |
| Mgi Id | MGI:1096643 | Doi | 10.1128/mcb.17.9.5236 |
| Citation | Yang Q, et al. (1997) Transient expression of a winged-helix protein, MNF-beta, during myogenesis. Mol Cell Biol 17(9):5236-43 |
| abstractText | A novel winged-helix transcription factor, MNF-beta, is expressed coincidentally with cell cycle withdrawal and differentiation of skeletal myogenic cells. MNF-beta is closely related to the myocyte nuclear factor (MNF) protein previously described (now termed MNF-alpha), but expression of the two isoforms is differentially regulated, and they exhibit distinctive functional properties with respect to DNA binding in vitro and transcriptional regulatory activity in transient-transfection assays. A DNA sequence motif binding MNF-beta with high affinity was selected from a library of random oligonucleotides and was found to be similar to but distinct from the cognate binding site for HNF-3beta, a more distantly related winged-helix protein. The temporal pattern of MNF-beta expression and the presence of MNF binding motifs within conserved promoter elements of several genes that modulate cell cycle progression support a working hypothesis that MNF proteins may modulate proliferation of myogenic precursor cells during development and muscle regeneration. |
