| First Author | Endharti AT | Year | 2005 |
| Journal | Eur J Immunol | Volume | 35 |
| Issue | 1 | Pages | 86-97 |
| PubMed ID | 15593120 | Mgi Jnum | J:95260 |
| Mgi Id | MGI:3525757 | Doi | 10.1002/eji.200425340 |
| Citation | Endharti AT, et al. (2005) Galectin-1 supports survival of naive T cells without promoting cell proliferation. Eur J Immunol 35(1):86-97 |
| abstractText | Naive T cells do not proliferate but remain alive in vivo. In contrast, naive T cells rapidly die in an in vitro culture, suggesting that some factors that are present at the sites of naive T cell circulation in vivo but missing in the bovine serum-containing culture medium, are necessary for their survival. The present study was designed to search for such factors. By functional screening of the cDNA library from murine lymph node-derived stromal cells (LNS) that effectively support the survival of naive T cells, we found that nascent polypeptide-associated complex (alpha-NAC) promoted T cell survival. A conditioned medium derived from culture supernatant of Cos7 cells transfected with alpha-NAC gene supported T cell survival, indicating that alpha-NAC induced production of soluble factor(s) that were secreted into the medium. By examining the products that were cloned from a functional screening of the cDNA library from alpha-NAC-transfected NIH3T3 cells but were not detected in that from control vector-transfected cells, galectin-1 was found as a soluble factor in the conditioned medium of the LNS. Our study demonstrates the novel role of galectin-1 as a soluble factor that functions to maintain naive T cell survival without inducing cell proliferation. |
