| Primary Identifier | MGI:4354271 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Rb1 |
| Transmission | Germline | Strain of Origin | 129S6/SvEvTac |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A targeting vector targeted to exon 22 was used to substitute a bulky phenylalanine for asparagine at amino acid 750, which is predicted to sterically block access to the LXCXE binding cleft. Southern blotting confirmed successful recombination. Analysis of mutant fibroblasts confirmed that protein levels were equivalent to wild-type cells. Crossing with mice expressing cre recombinase in the germline removed a floxed neo included in the vector. |
