Primary Identifier | MGI:5812580 | Allele Type | Targeted |
Attribute String | Conditional ready, Inserted expressed sequence, Reporter | Gene | Igs2 |
Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
Is Recombinase | false | Is Wild Type | false |
molecularNote | The construct is designed to contain (from 5'to 3') an attB site, a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG), a loxP-flanked stop cassette (LSL), a myelocytomatosis oncogene (Myc) cDNA with a T58A mutation fused to an internal ribosome entry site (IRES) followed by a luciferase gene, a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE; to enhance the mRNA transcript stability), and a polyadenylation sequence. This construct was microinjected, along with the PhiC31 integrase mRNA, into the pronuclei of zygotes collected from the TARGATT mice on a FVB/N congenic background. TARGATT mice carry the H11 |