| First Author | Svendsen PC | Year | 1997 |
| Journal | Gene | Volume | 189 |
| Issue | 2 | Pages | 175-81 |
| PubMed ID | 9168124 | Mgi Jnum | J:40051 |
| Mgi Id | MGI:87390 | Doi | 10.1016/s0378-1119(96)00797-4 |
| Citation | Svendsen PC, et al. (1997) The mouse uracil-DNA glycosylase gene: isolation of cDNA and genomic clones and mapping ung to mouse chromosome 5. Gene 189(2):175-81 |
| abstractText | Uracil-DNA glycosylase (UDG) is the enzyme responsible for the first step in the base-excision repair pathway that specifically removes uracil from DNA. Here we report the isolation of the cDNA and genomic clones for the mouse uracil-DNA glycosylase gene (ung) homologous to the major placental uracil-DNA glycosylase gene (UNG) of humans. The complete characterization of the genomic organization of the mouse uracil-DNA glycosylase gene shows that the entire mRNA coding region for the 1.83-kb cDNA of the mouse ung gene is contained in an 8.2-kb SstI genomic fragment which includes six exons and five introns. The cDNA encodes a predicted uracil-DNA glycosylase (UDG) protein of 295 amino acids (33 kDa) that is highly similar to a group of UDGs that have been isolated from a wide variety of organisms. The mouse ung gene has been mapped to mouse chromosome 5 using fluorescence in situ hybridization (FISH). (C) 1997 Elsevier Science B.V. |
