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Allele : Gt(ROSA)26Sor<tm4(CAG-Irf3*S388D*S390D)Evdr> gene trap ROSA 26, Philippe Soriano; targeted mutation 4, Evan Rosen
Primary Identifier  MGI:6695703 Allele Type  Targeted
Attribute String  Conditional ready, Epitope tag, Inserted expressed sequence Gene  Gt(ROSA)26Sor
Transmission  Germline Strain of Origin  C57BL/6N
Is Recombinase  false Is Wild Type  false
molecularNote  The targeting vector contains from 5' to 3', a chicken beta-actin promoter, a loxP-flanked stop cassette, the 3XFlag-tagged mouse Irf3 sequence modified to carry S388D and S390D amino acid substitutions (IRF3-2D), a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE; to enhance the mRNA transcript stability), and a bovine growth hormone polyA signal (bGHpA). Therefore the final construct reads ROSA26::CAG::loxP-STOP-loxP::3XFlag-IRF3-2D::WPRE::bGHpA. The S388D and S390D mutations result in a constitutively active allele without the need for an external stimulus (ie. lipopolysaccharide, poly I:C).
  • mutations:
  • Insertion
  • synonyms:
  • IRF3-2D,
  • IRF3-2D
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