| First Author | Xie K | Year | 2012 |
| Journal | Sci Signal | Volume | 5 |
| Issue | 239 | Pages | ra63 |
| PubMed ID | 22932702 | Mgi Jnum | J:259516 |
| Mgi Id | MGI:6141940 | Doi | 10.1126/scisignal.2002922 |
| Citation | Xie K, et al. (2012) The complex of G protein regulator RGS9-2 and Gbeta(5) controls sensitization and signaling kinetics of type 5 adenylyl cyclase in the striatum. Sci Signal 5(239):ra63 |
| abstractText | Multiple neurotransmitter systems in the striatum converge to regulate the excitability of striatal neurons by activating several heterotrimeric guanine nucleotide-binding protein (G protein)-coupled receptors (GPCRs) that signal to the type 5 adenylyl cyclase (AC5), the key effector enzyme that produces the intracellular second messenger cyclic adenosine monophosphate (cAMP). Plasticity of cAMP signaling in the striatum is thought to play an essential role in the development of drug addiction. We showed that the complex of the ninth regulator of G protein signaling (RGS9-2) with the G protein beta subunit (Gbeta(5)) critically controlled signaling from dopamine and opioid GPCRs to AC5 in the striatum. RGS9-2/Gbeta(5) directly interacted with and suppressed the basal activity of AC5. In addition, the RGS9-2/Gbeta(5) complex attenuated the stimulatory action of Gbetagamma on AC5 by facilitating the GTPase (guanosine triphosphatase) activity of Galpha(o), thus promoting the formation of the inactive heterotrimer and inhibiting Gbetagamma. Furthermore, by increasing the deactivation rate of Galpha(i), RGS9-2/Gbeta(5) facilitated the recovery of AC5 from inhibition. Mice lacking RGS9 showed increased cAMP production and, upon withdrawal from opioid administration, enhanced sensitization of AC5. Our findings establish RGS9-2/Gbeta(5) complexes as regulators of three key aspects of cAMP signaling: basal activity, sensitization, and temporal kinetics of AC5, thus highlighting the role of this complex in regulating both inhibitory and stimulatory GPCRs that shape cAMP signaling in the striatum. |
