| First Author | Chaudhari A | Year | 2015 |
| Journal | Mol Cell Biol | Volume | 35 |
| Issue | 19 | Pages | 3258-73 |
| PubMed ID | 26169833 | Mgi Jnum | J:228227 |
| Mgi Id | MGI:5705689 | Doi | 10.1128/MCB.00471-15 |
| Citation | Chaudhari A, et al. (2015) p110alpha Hot Spot Mutations E545K and H1047R Exert Metabolic Reprogramming Independently of p110alpha Kinase Activity. Mol Cell Biol 35(19):3258-73 |
| abstractText | The phosphatidylinositol-4,5-bisphosphate 3-kinase (PI3K) catalytic subunit p110alpha is the most frequently mutated kinase in human cancer, and the hot spot mutations E542K, E545K, and H1047R are the most common mutations in p110alpha. Very little is known about the metabolic consequences of the hot spot mutations of p110alpha in vivo. In this study, we used adenoviral gene transfer in mice to investigate the effects of the E545K and H1047R mutations on hepatic and whole-body glucose metabolism. We show that hepatic expression of these hot spot mutations results in rapid hepatic steatosis, paradoxically accompanied by increased glucose tolerance, and marked glycogen accumulation. In contrast, wild-type p110alpha expression does not lead to hepatic accumulation of lipids or glycogen despite similar degrees of upregulated glycolysis and expression of lipogenic genes. The reprogrammed metabolism of the E545K and H1047R p110alpha mutants was surprisingly not dependent on altered p110alpha lipid kinase activity. |
