| First Author | Shionoya T | Year | 2015 |
| Journal | BMC Dev Biol | Volume | 15 |
| Pages | 6 | PubMed ID | 25613821 |
| Mgi Jnum | J:218160 | Mgi Id | MGI:5616936 |
| Doi | 10.1186/s12861-015-0055-z | Citation | Shionoya T, et al. (2015) Distinct expression and localization of the type II diacylglycerol kinase isozymes inverted question mark, inverted question mark and inverted question mark in the mouse reproductive organs. BMC Dev Biol 15(1):6 |
| abstractText | BackgroundWe have revealed that the type II diacylglycerol kinases (DGKs) inverted question mark, inverted question mark and inverted question mark were expressed in the testis and ovary. However, these enzymes inverted question mark functions in the reproductive organs remain unknown.ResultsIn this study, we first identified the expression sites of type II DGKs in the mouse reproductive organs in detail. Reverse transcription-polymerase chain reaction and Western blotting confirmed that DGK inverted question mark2 (splicing variant 2) but not DGK inverted question mark1 (splicing variant 1) and DGK inverted question mark were expressed in the testis, ovary and uterus. DGK inverted question mark1 (splicing variant 1) but not DGK inverted question mark2 (splicing variant 2) was strongly detected in the ovary and uterus. Interestingly, we found that a new alternative splicing product of the DGK inverted question mark gene, DGK inverted question mark3, which lacks exon 26 encoding 31 amino acid residues, was expressed only in the testis. Moreover, we investigated the distribution of type II DGKs in the testis, ovary and uterus through in situ hybridization. DGK inverted question mark2 was distributed in the primary spermatocytes of the testis and ovarian follicles. DGK inverted question mark1 was distributed in the oviductal epithelium of the ovary and the luminal epithelium of the uterus. Intriguingly, DGK inverted question mark3 was strongly expressed in the secondary spermatocytes and round spermatids of the testis. DGK inverted question mark was distributed in the primary and secondary spermatocyte of the testis.ConclusionThese results indicate that the expression patterns of the type II DGK isoforms inverted question mark2, inverted question mark1, inverted question mark3 and inverted question mark differ from each other, suggesting that these DGK isoforms play specific roles in distinct compartments and developmental stages of the reproductive organs, especially in the processes of spermatogenesis and oocyte maturation. |
