| First Author | Powers GD | Year | 1994 |
| Journal | Cell Immunol | Volume | 153 |
| Issue | 2 | Pages | 298-311 |
| PubMed ID | 7509723 | Mgi Jnum | J:16653 |
| Mgi Id | MGI:64721 | Doi | 10.1006/cimm.1994.1030 |
| Citation | Powers GD, et al. (1994) Expression and functional analysis of murine B7 delineated by a novel monoclonal antibody. Cell Immunol 153(2):298-311 |
| abstractText | The B cell surface molecule designated B7 has been shown to be expressed by activated human B cells and monocytes and to be a ligand for the CD28 and CTLA-4 molecules on T cells. B7/CD28 interactions can provide a second signal to T cells (in addition to occupancy of the T cell antigen receptor) that is needed for T cell activation. COS cells transfected with the mouse homologue of B7 have been demonstrated to provide a stimulatory signal to murine and human T cells. In this report we describe a rat anti-mouse B7 mAb designated 1G10. Scatchard and/or FACS analyses utilizing 1G10 demonstrated that B7 was not expressed on resting splenic T cells or B cells, but could be induced at high levels on B cells cocultured with a syngeneic I-Ak-restricted autoreactive T cell hybridoma. Furthermore, activation of B cells with dibutyryl-cAMP (db-cAMP), a second messenger for class II MHC signaling, or with LPS induced the expression of B7 and the two agents showed additive effects. In contrast to B cells, freshly isolated mouse peritoneal macrophages constitutively expressed B7. Antibody-blocking experiments indicated that anti-B7 antibody partially inhibited T cell proliferative responses to primary antigenic stimulation but had no effect on the responses of previously activated T cells to antigenic restimulation. |
