| First Author | Cassano S | Year | 2014 |
| Journal | Metabolism | Volume | 63 |
| Issue | 10 | Pages | 1272-9 |
| PubMed ID | 25060689 | Mgi Jnum | J:228103 |
| Mgi Id | MGI:5705211 | Doi | 10.1016/j.metabol.2014.06.010 |
| Citation | Cassano S, et al. (2014) Leptin modulates autophagy in human CD4+CD25- conventional T cells. Metabolism 63(10):1272-9 |
| abstractText | OBJECTIVE: In this report we show that the adipocytokine leptin directly modulates autophagy in human CD4(+)CD25(-) conventional (Tconv) T cells. RESULTS: In vitro treatment with recombinant human leptin determined an inhibition of autophagy during T cell receptor (TCR) stimulation, and this phenomenon was dose- and time-dependent. The events were secondary to the activation of the mammalian-target of rapamycin (mTOR)-pathway induced by leptin, as testified by its reversion induced by mTOR inhibition with rapamycin. At molecular level these phenomena associated with Bcl-2 up-regulation and its interaction with Beclin-1, whose complex exerts a negative effect on autophagy. MATERIALS/METHODS: The impact of leptin on autophagy of Tconv cells was determined at biochemical level by western blotting and by flow cytometry; the interaction between BCL-2 and Beclin-1 by co-immunoprecipitation assays. CONCLUSIONS: Our results, suggest that in unconditioned, freshly-isolated human Tconv cells, autophagy and proliferation are controlled by leptin during TCR-engagement, and that both phenomena occur alternatively indicating a balance between these processes during immune activation. |
