First Author | Kelly SL | Year | 1994 |
Journal | Biochem J | Volume | 302 ( Pt 2) |
Pages | 347-53 | PubMed ID | 7545952 |
Mgi Jnum | J:20122 | Mgi Id | MGI:68236 |
Doi | 10.1042/bj3020347 | Citation | Kelly SL, et al. (1994) Arylamine N-acetyltransferase in Balb/c mice: identification of a novel mouse isoenzyme by cloning and expression in vitro. Biochem J 302(Pt 2):347-53 |
abstractText | Three genes encoding arylamine N-acetyltransferase were identified in Balb/c mice. All three genes were cloned from genomic DNA, sequenced and expressed in a bacterial expression system. Two of the genes corresponded to Nat-1 and Nat-2 which have been previously identified in A/J and C57B1/6 strains of mice (Martell et al., 1991). The new gene, designated Nat-3, can be distinguished from the other mouse Nat genes both by specific amplification using PCR and by restriction-endonuclease digestion. The products of all three genes are demonstrated to catalyse acetylation of aminofluorene and anisidine following expression in Escherichia coli. |