| First Author | Oliveira JF | Year | 2011 |
| Journal | Cereb Cortex | Volume | 21 |
| Issue | 4 | Pages | 806-20 |
| PubMed ID | 20739479 | Mgi Jnum | J:181824 |
| Mgi Id | MGI:5314216 | Doi | 10.1093/cercor/bhq154 |
| Citation | Oliveira JF, et al. (2011) Rodent cortical astroglia express in situ functional P2X7 receptors sensing pathologically high ATP concentrations. Cereb Cortex 21(4):806-20 |
| abstractText | ATP is an important neuronal and astroglial signaling molecule in the brain. In the present study, brain slices were prepared from the prefrontal cortex (PFC) of Wistar rats and 2 lines of mice. P2X receptor immunoreactivity was colocalized with astro- and microglial but not neuronal markers. Whole-cell patch-clamp recordings showed that, in astroglial cells, dibenzoyl-ATP (BzATP) and ATP caused inward currents, near the resting membrane potential. The inactivity of alpha,beta-methylene ATP, as well as the potency increases of BzATP and ATP in a low divalent cation (X(2)(+))-containing superfusion medium suggested the involvement of P2X receptors. This idea was corroborated by the inhibition of these current responses by PPADS, Brilliant Blue G, A 438079, and calmidazolium. Ivermectin, trinitrophenyl-adenosine-5'-triphosphate, and cyclopentyl-dipropylxanthine did not alter the agonist effects. The reversal potential of BzATP was near 0 mV, indicating the opening of cationic receptor channels. In a low X(2)(+) superfusion medium, ATP-induced current responses in PFC astroglial cells of wild-type mice but not of the P2X knockouts. Hence, cortical astroglia of rats and mice possess functional P2X receptors. These receptors may participate in necrotic/apoptotic or proliferative reactions after stimulation by large quantities of ATP released by central nervous system injury. |
