Primary Identifier | MGI:5514399 | Allele Type | Targeted |
Attribute String | Conditional ready, No functional change | Gene | Kdm1a |
Transmission | Germline | Strain of Origin | 129S1/Sv-Oca2<+> Tyr<+> Kitl<+> |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A targeting vector was designed to insert a single loxP site followed by a frt-flanked neomycin resistance (neo) cassette upstream of exon 5, and a second loxP site downstream of exon 6. Exons 5 and 6 encode a flavin adenine dinucleotide binding site and the N-terminal portion of the amine oxidase domain, both required for enzymatic activity. FLPe-mediated recombination removed the selection cassette. |