| First Author | LoMastro GM | Year | 2022 |
| Journal | Elife | Volume | 11 |
| PubMed ID | 35969030 | Mgi Jnum | J:329155 |
| Mgi Id | MGI:7341157 | Doi | 10.7554/eLife.80643 |
| Citation | LoMastro GM, et al. (2022) PLK4 drives centriole amplification and apical surface area expansion in multiciliated cells. Elife 11:e80643 |
| abstractText | Multiciliated cells (MCCs) are terminally differentiated epithelia that assemble multiple motile cilia used to promote fluid flow. To template these cilia, MCCs dramatically expand their centriole content during a process known as centriole amplification. In cycling cells, the master regulator of centriole assembly Polo-like kinase 4 (PLK4) is essential for centriole duplication; however recent work has questioned the role of PLK4 in centriole assembly in MCCs. To address this discrepancy, we created genetically engineered mouse models and demonstrated that both PLK4 protein and kinase activity are critical for centriole amplification in MCCs. Tracheal epithelial cells that fail centriole amplification accumulate large assemblies of centriole proteins and do not undergo apical surface area expansion. These results show that the initial stages of centriole assembly are conserved between cycling cells and MCCs and suggest that centriole amplification and surface area expansion are coordinated events. |
