| First Author | Hara S | Year | 1999 |
| Journal | Biochim Biophys Acta | Volume | 1445 |
| Issue | 2 | Pages | 237-43 |
| PubMed ID | 10320777 | Mgi Jnum | J:55057 |
| Mgi Id | MGI:1337188 | Doi | 10.1016/s0167-4781(99)00048-2 |
| Citation | Hara S, et al. (1999) Molecular cloning of cDNAs encoding hypoxia-inducible factor (HIF)-1alpha and -2alpha of bovine arterial endothelial cells. Biochim Biophys Acta 1445(2):237-43 |
| abstractText | Hypoxia-inducible factor (HIF)-1alpha and -2alpha are two basic helix-loop-helix/PAS domain transcriptional factors that mediate hypoxia-induced gene expression. We found that bovine arterial endothelial cells (BAEC) expressed both HIF-1alpha and -2alpha by RT-PCR and then isolated cDNAs encoding these two transcriptional factors of BAEC. The deduced amino acid sequences of both HIF-1alpha and -2alpha showed high homologies among mammalian species. Northern blot analysis indicated that the mRNAs for HIF-1alpha and -2alpha from BAEC showed a size of approx. 5.5 and 6.2 kilobases, respectively and that both mRNAs were constitutively expressed and not induced by hypoxia in BAEC. |
