| First Author | Cambray S | Year | 2009 |
| Journal | Mol Cell Biol | Volume | 29 |
| Issue | 3 | Pages | 726-35 |
| PubMed ID | 19015237 | Mgi Jnum | J:145560 |
| Mgi Id | MGI:3835232 | Doi | 10.1128/MCB.01180-08 |
| Citation | Cambray S, et al. (2009) Protein kinase KIS localizes to RNA granules and enhances local translation. Mol Cell Biol 29(3):726-35 |
| abstractText | The regulation of mRNA transport is a fundamental process for cytoplasmic sorting of transcripts and spatially controlled translational derepression once properly localized. There is growing evidence that translation is locally modulated as a result of specific synaptic inputs. However, the underlying molecular mechanisms that regulate this translational process are just emerging. We show that KIS, a serine/threonine kinase functionally related to microtubule dynamics and axon development, interacts with three proteins found in RNA granules: KIF3A, NonO, and eEF1A. KIS localizes to RNA granules and colocalizes with the KIF3A kinesin and the beta-actin mRNA in cultured cortical neurons. In addition, KIS is found associated with KIF3A and 10 RNP-transported mRNAs in brain extracts. The results of knockdown experiments indicate that KIS is required for normal neurite outgrowth. More important, the kinase activity of KIS stimulates 3' untranslated region-dependent local translation in neuritic projections. We propose that KIS is a component of the molecular device that modulates translation in RNA-transporting granules as a result of local signals. |
