| First Author | Han YE | Year | 2019 |
| Journal | Exp Neurobiol | Volume | 28 |
| Issue | 2 | Pages | 183-215 |
| PubMed ID | 31138989 | Mgi Jnum | J:345046 |
| Mgi Id | MGI:7581104 | Doi | 10.5607/en.2019.28.2.183 |
| Citation | Han YE, et al. (2019) Tweety-homolog (Ttyh) Family Encodes the Pore-forming Subunits of the Swelling-dependent Volume-regulated Anion Channel (VRAC(swell)) in the Brain. Exp Neurobiol 28(2):183-215 |
| abstractText | In the brain, a reduction in extracellular osmolality causes water-influx and swelling, which subsequently triggers Cl(-)- and osmolytes-efflux via volume-regulated anion channel (VRAC). Although LRRC8 family has been recently proposed as the pore-forming VRAC which is activated by low cytoplasmic ionic strength but not by swelling, the molecular identity of the pore-forming swelling-dependent VRAC (VRAC(swell)) remains unclear. Here we identify and characterize Tweety-homologs (TTYH1, TTYH2, TTYH3) as the major VRAC(swell) in astrocytes. Gene-silencing of all Ttyh1/2/3 eliminated hypo-osmotic-solution-induced Cl(-) conductance (I(Cl,swell)) in cultured and hippocampal astrocytes. When heterologously expressed in HEK293T or CHO-K1 cells, each TTYH isoform showed a significant I(Cl,swell) with similar aquaporin-4 dependency, pharmacological properties and glutamate permeability as I(Cl,swell) observed in native astrocytes. Mutagenesis-based structure-activity analysis revealed that positively charged arginine residue at 165 in TTYH1 and 164 in TTYH2 is critical for the formation of the channel-pore. Our results demonstrate that TTYH family confers the bona fide VRAC(swell) in the brain. |
