| First Author | Raina D | Year | 2005 |
| Journal | J Biol Chem | Volume | 280 |
| Issue | 12 | Pages | 11147-51 |
| PubMed ID | 15657060 | Mgi Jnum | J:196110 |
| Mgi Id | MGI:5486558 | Doi | 10.1074/jbc.M413787200 |
| Citation | Raina D, et al. (2005) c-Abl tyrosine kinase regulates caspase-9 autocleavage in the apoptotic response to DNA damage. J Biol Chem 280(12):11147-51 |
| abstractText | Activation of the initiator caspase-9 is essential for induction of apoptosis by developmental signals, oncogenic transformation, and genotoxic stress. The c-Abl tyrosine kinase is also involved in the apoptotic response to DNA damage. The present results demonstrate that c-Abl binds directly to caspase-9. We show that c-Abl phosphorylates caspase-9 on Tyr-153 in vitro and in cells treated with DNA damaging agents. Moreover, inhibition of c-Abl with STI571 blocked DNA damage-induced autoprocessing of caspase-9 to the p35 subunit and activation of caspase-3. Caspase-9(Y153F) also attenuated DNA damage-induced processing of caspase-9 to p35, activation of caspase-3, and apoptosis. These findings indicate that caspase-9 autoprocessing is regulated by c-Abl in the apoptotic response to genotoxic stress. |
