| First Author | Chen J | Year | 1994 |
| Journal | Immunity | Volume | 1 |
| Issue | 1 | Pages | 65-72 |
| PubMed ID | 7889400 | Mgi Jnum | J:25008 |
| Mgi Id | MGI:72723 | Doi | 10.1016/1074-7613(94)90010-8 |
| Citation | Chen J, et al. (1994) Generation of normal T and B lymphocytes by c-jun deficient embryonic stem cells. Immunity 1(1):65-72 |
| abstractText | To determine the potential roles of c-jun in lymphocyte development, we generated somatic chimeric mice by injecting homozygous c-jun mutant embryonic stem (ES) cells into blastocysts from recombination activating gene-2 (RAG-2)-deficient mice. Chimeric mice had poor restoration of thymocytes, but contained substantial numbers of mature T and B lymphocytes in the periphery. Stimulation of c-jun-/- B cells resulted in normal levels of proliferation and immunoglobulin secretion. Likewise, stimulation of c-jun-/- T cells resulted in essentially normal levels of IL-2R alpha expression, IL-2 secretion, and proliferation. We further showed that the relatively normal activation responses of the c-jun-/- T cells probably results from the fact that other members of the Jun family contribute to the bulk of the activator protein-1 (AP-1) complexes in normal T cells and, as a result, AP-1 complexes are found at relatively normal levels in c-jun-/- T cells. |
