First Author | Tanner B | Year | 1993 |
Journal | Biochim Biophys Acta | Volume | 1171 |
Issue | 3 | Pages | 319-20 |
PubMed ID | 8424957 | Mgi Jnum | J:3921 |
Mgi Id | MGI:52426 | Doi | 10.1016/0167-4781(93)90074-n |
Citation | Tanner B, et al. (1993) Molecular cloning of a mouse extracellular signal regulated kinase (erk-1). Biochim Biophys Acta 1171(3):319-20 |
abstractText | A complementary DNA has been cloned and sequenced for the mouse extracellular signal regulated kinase (erk-1). The nucleotide sequence of the 1.7 kb mouse erk-1 cDNA clone (merk-1-34) is 93.5% identical to the published rat DNA sequence (Boulton et al. (1990) Science 249, 64-67). The mouse clone, as with all other reported sequences, did not contain an in-frame translation initiation codon and, therefore, does not appear to be full-length. Attempts to generate cDNA sequence upstream of the 5' end of merk-1-34 using thermostable reverse transcriptase and chemical melting of the mRNA (methylmercury hydroxide) were unsuccessful. |