| First Author | Madlener M | Year | 1997 |
| Journal | Gene | Volume | 202 |
| Issue | 1-2 | Pages | 75-81 |
| PubMed ID | 9427548 | Mgi Jnum | J:44616 |
| Mgi Id | MGI:1100634 | Doi | 10.1016/s0378-1119(97)00456-3 |
| Citation | Madlener M, et al. (1997) cDNA cloning and expression of the gene encoding murine stromelysin-2 (MMP-10). Gene 202(1-2):75-81 |
| abstractText | Recently, we demonstrated a biphasic induction of the epithelial broad-spectrum matrix metalloproteinase (MMP) stromelysin-2 during cutaneous wound healing. Now we have generated a murine wound cDNA libary and have used it to isolate the putative cDNA of this murine matrix metalloproteinase. The predicted sequence of the protein shows 76 and 89% identity with its human and rat analogues, respectively. Stromelysin-2 and stromelysin-1 transcripts were both detected at very low levels in the lung and the heart of adult Balb/c mice, whereas stromelysin-2 mRNA expression alone was found at comparatively high levels in the small intestine, a tissue characterized by continuous epithelial renewal. Recombinant forms of murine stromelysin-1 and -2 produced in transfected COS cells were secreted and could be induced to undergo autocatalytic processing by addition of the organomercurial salt 4-aminophenylmercuric acetate (APMA). |
