| First Author | Maric M | Year | 2001 |
| Journal | Science | Volume | 294 |
| Issue | 5545 | Pages | 1361-5 |
| PubMed ID | 11701933 | Mgi Jnum | J:72708 |
| Mgi Id | MGI:2153425 | Doi | 10.1126/science.1065500 |
| Citation | Maric M, et al. (2001) Defective Antigen Processing in GILT-Free Mice. Science 294(5545):1361-5 |
| abstractText | Processing of proteins for major histocompatibility complex (MHC) class II-restricted presentation to CD4-positive T lymphocytes occurs after they are internalized by antigen-presenting cells (APCs). Antigenic proteins frequently contain disulfide bonds, and their reduction in the endocytic pathway facilitates processing. In humans, a gamma interferon-inducible lysosomal thiol reductase (GILT) is constitutively present in late endocytic compartments of APCs. Here, we identified the mouse homolog of GILT and generated a GILT knockout mouse. GILT facilitated the processing and presentation to antigen-specific T cells of protein antigens containing disulfide bonds. The response to hen egg lysozyme, a model antigen with a compact structure containing four disulfide bonds, was examined in detail. |
