| Primary Identifier | MGI:4437237 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Ext1 |
| Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | An inverted loxP site was introduced upstream to exon 2 in a genomic fragment containing the Ext1 gene. The fragment was then subcloned into a vector upstream of the forward-facing loxP-flanked neomycin resistance (Neo) cassette. The linearized targeting vector was electroporated into R1 ES cells. Homozygous Ext1tm1Kjns mice were crossed to Prxx1-cre transgenic mice that express cre in the germline. This results in two consequences, either excision of neo only (tm1.1) or neo-excision and exon 2 inversion (tm1.2). Homozygous exon2-inverted animals display embryonic lethality. |
