Primary Identifier | MGI:7384385 | Allele Type | Endonuclease-mediated |
Attribute String | Null/knockout | Gene | Mpnd |
Inheritance Mode | Not Specified | Strain of Origin | C57BL/6NJ |
Is Recombinase | false | Is Wild Type | false |
Project Collection | IMPC |
molecularNote | This allele was generated at The Jackson Laboratory by electroporating Cas9 protein and 2 guide sequences TGCCTGGACCAACCGCCAGA and ATCAGGATTCAGGATTCCCC, which resulted in a 389 bp deletion beginning at Chromosome 17 position 56,010,881 bp and ending after 56,011,269 bp (GRCm38/mm10). This mutation deletes ENSMUSE00001271227 (exon 4) and 265 bp of flanking intronic sequence including the splice acceptor and donor and is predicted to cause a change of amino acid sequence after residue 167 and early truncation 33 amino acids later. |