| First Author | Arnoldussen YJ | Year | 2015 |
| Journal | Cytokine | Volume | 73 |
| Issue | 1 | Pages | 128-37 |
| PubMed ID | 25748835 | Mgi Jnum | J:319077 |
| Mgi Id | MGI:6862607 | Doi | 10.1016/j.cyto.2015.01.032 |
| Citation | Arnoldussen YJ, et al. (2015) Involvement of IL-1 genes in the cellular responses to carbon nanotube exposure. Cytokine 73(1):128-37 |
| abstractText | The interleukin-1 (IL-1) family has been implicated in cellular responses to nanoparticles including carbon nanotubes (CNTs). IL-1alpha and beta are key proinflammatory cytokines important in inflammatory and oxidative stress responses. The aim of this study was to characterize the role of IL-1 in cellular responses of CNTs in cells from IL-1alpha/beta wild type (IL1-WT) mice and cells with reduced inflammatory potential from IL-1alpha/beta deficient (IL1-KO) mice. Two multi-walled CNTs, CNT-1 containing long and thick fibers and CNT-2 containing short and thin fibers, were compared to UICC crocidolite asbestos fibers. Upon CNT exposure toxicity and apoptosis were affected differently in IL1-WT and IL1-KO cells. Upregulation of TNFalpha and IL-1alpha mRNA expression in IL1-WT cells was dependent on the type of CNT. On the contrary precursor IL-1alpha protein was downregulated after 24h. The mitogen-activated protein kinase (MAPK) c-Jun N-terminal kinase (JNK) was activated in IL1-KO cells and regulated by CNTs, whereas no significant changes of extracellular regulated kinase (ERK) were observed when comparing IL1-WT and IL1-KO cells. In summary, the results presented here indicate that IL-1 contributes to the cellular and molecular effects of CNT exposure and that the type of CNT has an important effect on the cellular response. |
