Primary Identifier | MGI:5586715 | Allele Type | Targeted |
Attribute String | Humanized sequence | Gene | Cst3 |
Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
Is Recombinase | false | Is Wild Type | false |
molecularNote | The entire human cystatin C gene with a leucine 68 to glutamine (L68Q) single point mutation in exon 2 (without the human promoter) was inserted downstream of the mouse cystatin C gene promoter. ELISA confirmed the human protein in epididymal luminal fluid, and brain and testis lysates and Western blot confirmed expression in epididymal luminal fluid. |