| First Author | Girling R | Year | 1993 |
| Journal | Nature | Volume | 362 |
| Issue | 6415 | Pages | 83-7 |
| PubMed ID | 8446173 | Mgi Jnum | J:17080 |
| Mgi Id | MGI:65134 | Doi | 10.1038/362083a0 |
| Citation | Girling R, et al. (1993) A new component of the transcription factor DRTF1/E2F [published erratum appears in Nature 1993 Sep 30;365(6445):468]. Nature 362(6415):83-7 |
| abstractText | Transcription factor DRTF1/E2F coordinates events in the cell cycle with transcription by its cyclical interactions with important regulators of cellular proliferation like the retinoblastoma tumour-suppressor gene product (Rb) and the Rb-related protein, p107 (refs 1-8). DRTF1/E2F binding sites occur in the control regions of genes involved in proliferation, and both Rb and p107 repress the capacity of DRTF1/E2F to activate transcription (refs 11, 12; M. Zamanian and N.B.L.T., manuscript submitted). Mutant Rb proteins isolated from tumour cells are unable to bind DRTF1/E2F (refs 11-13), and certain viral oncoproteins, such as adenovirus E1A, sequester Rb and p107 in order to free active DRTF1/E2F (refs 5, 11, 12, 14, 15). Here we report the isolation of a complementary DNA encoding DRTF1-polypeptide-1 (DP-1), a major sequence-specific binding protein that is present in DRTF1/E2F, including Rb- and p107-associated DRTF1/E2F. The DNA-binding domain of DP-1 contains a region that resembles that of E2F-1 (refs 16, 17), and recognizes the same sequence. DRTF1/E2F thus appears to contain at least two sequence-specific DNA-binding proteins. |
