| First Author | Yoshioka S | Year | 2014 |
| Journal | Stem Cells | Volume | 32 |
| Issue | 3 | Pages | 730-40 |
| PubMed ID | 24115241 | Mgi Jnum | J:206765 |
| Mgi Id | MGI:5551953 | Doi | 10.1002/stem.1555 |
| Citation | Yoshioka S, et al. (2014) CCAAT/Enhancer-Binding Protein beta Expressed by Bone Marrow Mesenchymal Stromal Cells Regulates Early B-Cell Lymphopoiesis. Stem Cells 32(3):730-40 |
| abstractText | The transcription factor CCAAT/enhancer-binding protein beta (C/EBPbeta) regulates the differentiation of a variety of cell types. Here, the role of C/EBPbeta expressed by bone marrow mesenchymal stromal cells (BMMSCs) in B-cell lymphopoiesis was examined. The size of the precursor B-cell population in bone marrow was reduced in C/EBPbeta-knockout (KO) mice. When bone marrow cells from C/EBPbeta-KO mice were transplanted into lethally irradiated wild-type (WT) mice, which provide a normal bone marrow microenvironment, the size of the precursor B-cell population was restored to a level equivalent to that generated by WT bone marrow cells. In coculture experiments, BMMSCs from C/EBPbeta-KO mice did not support the differentiation of WT c-Kit(+) Sca-1(+) Lineage(-) hematopoietic stem cells (KSL cells) into precursor B cells, whereas BMMSCs from WT mice did. The impaired differentiation of KSL cells correlated with the reduced production of CXCL12/stromal cell-derived factor-1 by the cocultured C/EBPbeta-deficient BMMSCs. The ability of C/EBPbeta-deficient BMMSCs to undergo osteogenic and adipogenic differentiation was also defective. The survival of leukemic precursor B cells was poorer when they were cocultured with C/EBPbeta-deficient BMMSCs than when they were cocultured with WT BMMSCs. These results indicate that C/EBPbeta expressed by BMMSCs plays a crucial role in early B-cell lymphopoiesis. Stem Cells 2014;32:730-740. |
