| Primary Identifier | MGI:6790656 | Allele Type | Targeted |
| Attribute String | Conditional ready | Gene | Star |
| Transmission | Germline | Strain of Origin | 129 x C57BL/6 |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A EUCOMM targeting vector was used to generate a conditional allele. The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exons 2-5 at position 26301547. The critical exons 2 to 5 are thus flanked by loxP sites. The lacZ and neo cassette were removed through subsequent flp-mediated recombination. |
