| First Author | Arikan MC | Year | 2002 |
| Journal | Brain Res Mol Brain Res | Volume | 101 |
| Issue | 1-2 | Pages | 109-21 |
| PubMed ID | 12007838 | Mgi Jnum | J:76910 |
| Mgi Id | MGI:2180593 | Doi | 10.1016/s0169-328x(02)00178-x |
| Citation | Arikan MC, et al. (2002) Modulation of the membrane-binding projection domain of tau protein: splicing regulation of exon 3. Brain Res Mol Brain Res 101(1-2):109-21 |
| abstractText | Tau is a microtubule-associated protein whose transcript undergoes complex regulated splicing in the mammalian nervous system. The N-terminal domain of the protein interacts with the axonal membrane, and is modulated by differential inclusion of exons 2 and 3. These two tau exons are alternatively spliced cassettes, in which exon 3 never appears independently of exon 2. Previous work with tau minigene constructs indicated that exon 3 is intrinsically suboptimal and its primary regulator is a weak branch point. In this study, we confirm the role of the weak branch point in the regulation of exon 3 but also show that the exon is additionally regulated by a combination of exonic enhancers and silencers. Furthermore, we demonstrate that known splicing regulators affect the ratio of exon 3 isoforms, Lastly, we tentatively pinpoint the site of action of several splicing factors which regulate tau exon 3. |
